Microalgae Expression System

In the past decades, transgenic plants system has attracted the attention of pharmaceutical company for biopharmaceuticals production. Microalgae, also called microphytes, are polyphyletic groups ranging from prokaryotes to eukaryotes, such as Synechococcus and Chlamydomonas. It has been used for the production of food, food additives, and cosmetics for a long time. Chlamydomonas reinhardtii is the most popular microalgae used in research studies. As eukaryotes, algae can perform posttranslational modifications such as glycosylation, which are often necessary for protein function. Besides, microalgae are toxin free and animal free in production of recombinant protein, which makes microalgae an advantage in biosecurity. With the posttranslational modifications and reduced pathogenic compounds contamination, algae become more and more attractive for bio-pharmaceuticals production.

Microalgae are ideal platform for large-scale production of high-value products because they are fast-growing solar-powered biofactories with minimal nutritional needs. Many of these species are generally regarded as safe (GRAS) for human consumption and have been farmed commercially to produce a variety of biological products relevant to human or animal health. Recombinant proteins such as protein vaccines, therapeutic antibodies, and industrial enzymes can also be produced in microalgae, where low-cost production can greatly impact applicability.

Chlamydomonas reinhardtii, as the unicellular green microalga, is the one of model organisms. In the past decades, it had been used in production of enzymes, antigenic peptides or antibodies and the application of algal system has gaining the interest in valuable biopharmaceutical production. Besides, target protein allowed to express in both nuclear and chloroplast genomes of Chlamydomonas.

Services

Profacgen provides you with a one-stop recombinant protein production service from gene cloning to protein expression and purification in Chlamydomonas reinhardtii system. We have specialists in transgenic microalgae and protein expression and purification. During the service process, we will focus on the following factors:

• Codon bias in nuclear genomes, and codon bias in organelles,
• Promoters and regulatory elements that control transgenes (such as optimization of chloroplast promoters and untranslated regions (UTRs); fusion of the 16s ribosomal promoter with the atpA 5' UTR; fusion of the hsp70 promoter with rbcs2 promoter,etc.)
• Selectable markers (humanized enhanced GFP (eGFP), etc.)

Profacgen has accumulated lots of experience in protein expression. Our professional technical team can provide customers with high-quality microalgae expression system and many related featured services. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.

Reference

1. Barrera, D.J.; Mayfield, S.P. High-value Recombinant Protein Production in Microalgae. Handbook of Microalgal Culture. 2013.