Hansenula polymorpha Protein Expression Systems

The methylotrophic yeast Hansenula polymorpha (also known as Ogataea polymorpha) has emerged as a powerful platform for recombinant protein production due to its unique biological and technical advantages.

• High Protein Secretion Capacity: Unlike traditional yeast systems like Saccharomyces cerevisiae, H. polymorpha efficiently secretes heterologous proteins into the culture medium, simplifying downstream purification.
• Post-Translational Modifications: It performs human-like glycosylation patterns, enhancing the functionality and stability of therapeutic proteins.
• Strong Promoters and High Yield: The methanol-inducible MOX (methanol oxidase) and FMD (formate dehydrogenase) promoters enable tightly regulated, high-level protein expression under optimized fermentation conditions.
• Thermotolerance and Scalability: Its ability to grow at elevated temperatures (up to 50°C) accelerates fermentation and reduces contamination risks, while its adaptability to high-cell-density cultures ensures cost-effective industrial-scale production.
• Minimal Hyperglycosylation: Unlike Pichia pastoris, H. polymorpha rarely adds excessive mannose residues to glycoproteins, reducing immunogenicity risks for clinical applications.

This versatile system is widely employed across biotechnology and pharmaceutical industries:

• Therapeutic Proteins: Production of monoclonal antibodies, cytokines (e.g., interferons), and blood-clotting factors with proper folding and bioactivity.
• Industrial Enzymes: High-yield synthesis of lipases, amylases, and cellulases for biofuels, food processing, and detergent formulations.
• Vaccine Development: Surface antigen expression for hepatitis B virus (HBV) and human papillomavirus (HPV) vaccines.
• Diagnostic Reagents: Manufacture of enzymatically active proteins for diagnostic kits, such as horseradish peroxidase (HRP) conjugates.
• Membrane Proteins: Efficient expression of challenging targets like G protein-coupled receptors (GPCRs) for structural studies.

We provide end-to-end solutions tailored to clients' needs:

• Strain Development: Custom engineering of H. polymorpha strains with optimized promoters (e.g., MOX, FMD), secretion signals, and glycoengineering for humanized glycosylation.
• Fermentation Optimization: High-cell-density fed-batch fermentation in bioreactors (up to 1,000 L scale) to maximize protein yields.
• Downstream Processing: Integrated purification workflows using affinity chromatography, ion exchange, and size-exclusion techniques to achieve >95% purity.
• Analytical Services: Comprehensive characterization via SDS-PAGE, Western blot, mass spectrometry, and activity assays.

With its superior secretion efficiency, scalability, and compatibility with complex proteins, Hansenula polymorpha is a robust alternative to bacterial and mammalian systems. Our expertise in strain engineering, process development, and quality control ensures rapid delivery of high-titer, bioactive proteins for research, diagnostics, and therapeutics. Partner with us to leverage this cutting-edge platform for your next protein production challenge.