N6-methyladenosine, also called m6A, is a base modification behavior widely found on mRNA. The internal modification of mRNA is used to maintain the stability of mRNA. The current technical methods used to detect m6A include high-throughput sequencing, colorimetry, and LC-MS. Among them, LC-MS/MS and colorimetry can detect the overall m6A level of mRNA, while MeRIP-seq and miCLIP-seq(m6A individual-nucleotide-resolution cross-linking and immunoprecipitation combined with high-throughput sequencing) are high-throughput sequencing methods.
miCLIP-seq is to use the m6A antibody to enrich the m6A modification, and cooperate with the ultraviolet cross-linking technology to identify the m6A modification at the single-base level in the whole genome.
The process of miCLIP-seq:
Figure 1 The miCLIP protocol .
As a very important RNA epigenetic modification, how m6A cooperates with DNA and histone epigenetics to regulate gene expression requires further exploration. miCLIP is a highly sensitive tool that may be widely used in other epigenetic modifications that occur on RNA.
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