SERVICES

• Recombinant Protein or Antibody Production • Protein Expression Systems > Bacterial Expression Systems(E. coli / Bacillus) > Yeast Expression Systems(P pastoris / S cerevisiae) > Baculovirus-insect Cell Expression Systems > Mammalian Expression Systems(CHO / 293) • PEGylation Services • Stable Cell Line Construction for Protein Expression • Process Development • cGMP Manufacturing • QC and QA • Bacterial Manufacturing • Bacteria Expression • Yeast Expression • Mammalian Cell Expression • Insect Cell Expression • Animal-free Expression • Cell-free protein expression • Large-scale Protein Production • PEGylation Services • Basic Protein Characterization • Protein Labeling • Stable Cell Line Construction for Protein Expression • Protein Mutagenesis • Protein Interaction Analysis Services > Yeast Two-Hybrid Screening > Membrane-based Yeast Two-Hybrid Screening > Co-Immunoprecipitation (Co-IP) > Surface plasmon resonance (SPR) Service > Pull Down Assay > Bio-layer Interferometry (BLI) Service > CLIP-Seq Service > Protein Binding Site Mapping • Yeast Two-Hybrid Screening • Membrane-based Yeast Two-Hybrid Screening • Co-Immunoprecipitation (Co-IP) • Surface plasmon resonance (SPR) Service • Pull Down Assay • Protein Mutant Library Construction • Enzyme Target & Activator & Inhibitor Screening Service • GPCR Screening Service • Ion Channel Screening Service • Bio-layer Interferometry (BLI) Service • Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis > Electrophoretic Mobility Shift Assay (EMSA) Service > Chromatin Immunoprecipitation (ChIP) Assay Service > DNase I Footprinting Assay Service • Transporter Screening Service • Chromatin Immunoprecipitation (ChIP) Assay Service • Enzyme Activity Assay Service • DNase I Footprinting Assay Service • Protein Expression > Custom Lysate Preparation Service > C1 Expression System > Protein Co-expression Service in E. coli System > Protein Co-expression Service in Mammalian Cells • Protein Biotinylation Service • Custom Lysate Preparation Service • Immunogenicity Test Service > Anti-Drug Antibody (ADA) Evaluation Service > Neutralizing Antibody (NAb) Test Service • Anti-Drug Antibody (ADA) Evaluation Service • Neutralizing Antibody (NAb) Test Service • Thanksgiving Day Sale • C1 Expression System • Protein Conjugation Service on Beads • CLIP-Seq Service • Protein Binding Site Mapping • Quantum Dot Labeling • Protein Co-expression Service in E. coli System • Protein Co-expression Service in Mammalian Cells

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OUR PROMISE
Tel: 1-631-448-8149
Fax: 1-631-938-8127
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Stable Cell Line Construction for Protein Expression


When large amount of recombinant protein is required, stable expression line construction is recommended for reproducible and cost-effective protein production. Construction of a stable cell line usually takes 3 steps: delivery of the target gene into the host cell, screening of stable expression clones, and stability test.

Stable Cell Line Construction for Protein Expression

Features:

  •   High yield and reproducibility
  •   High cell line stability
  •   One-stop service
  •   Service fully customizable

At Profacgen, we use lentiviral vector for efficient gene delivery and to achieve high integration rate. Limited dilution and antibiotics screening are then applied to select for high-yield, genetically homogenous stable expression monoclones. When fluorescent protein is fused to the target protein, we can also use flow cytometry to screen for positive clones. Profacgen also offers the generation of Tet-ON inducible stable cell lines for inducible expression of your target gene. Doxycycline can then be used to induce the expression of your target gene.

To ensure integration of your target gene and stable/inducible expression of the protein, we can provide PCR, qRT-PCR, microscopy, flow cytometry analysis and stability test according to your specific requests.

Profacgen provides stable cell line construction service both for research use and for industrial production. We guarantee well-developed stable cell lines with robust and reproducible production of your target protein.

INQUIRY

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