Transporter Screening Service

Transporter Screening Service

Transporters are a group of specialized membrane proteins that power the translocation of numerous substances, including essential nutrients, ions, neurotransmitters, cellular waste and environmental toxins, across the cellular membrane. Many transporters have emerged as therapeutic targets for diseases such as depression, schizophrenia, cancer, and diabetes. Therefore, transporter screening for therapeutically effective and safe compounds has become very important in the discovery and development of transporter-targeted drugs.

Schematic Diagram of Transporters ^[1]

Profacgen provides specialized transporter panels and customized transporter screening services enabling you to identify novel transporter-targeted compounds. Our transporter panels include FDA transporter screening panel, monoamine transporter models and many other cell models. We employ robust transporter uptake and inhibition assays to perform quick and cost-effective screening of new molecular entities (NMEs) for their interactions with transporters, and offer you results with good signal-to-background ratio and Z’ scores.

Assays:

•   Electrophysiology
•   Radioactive flux detection
•   Fluorescence detection

Please feel free to contact us for more details about our customized transporter screening service.

Profacgen offers transporter targets that have great significance in clinical therapy, such as ATP binding cassette transporter (P-gp), neurotransmitter (SERT, DAT, NET), and solution carrier family transporter (SLC13). Our assay methods include radio-isotopic detection, fluorescence detection and ion flux detection.
See the instruction of transporter screening service. Find the targets list available in our screening service and the assay format we could provide to you.
Click here to download the Service Instruction.

References:

[1] G. Diallinas. Understanding Transporter Specifity and the Discrete Appearance of Channel-Like Gating Domains in Transporters. Front Pharmacol, 2014, 12 (5): 207.

SERVICES

• PROTAC
  • E3 ligase and target proteins development services
    • Custom Protein Ubiquitination Services
    • E3 ligase or target proteins expression and purification services
    • Novel E3 discovery
      • Gene sequencing and analysis
    • Protein engineering
      • Mutagenesis and Structural Modification
  • Ligand Discovery and Design Service
    • Custom Peptide and Compound Synthesis Service
    • Peptide Design for E3 Ligase or Target Protein
    • Small Molecule Ligands Screening
  • PROTAC in vitro evaluation
    • Cell Cytotoxicity Test
    • Degradation Assay and Stability Analysis
    • E3 Ligase Activity Assay
    • Permeability Assay
    • Ternary Complex Formation
    • Ubiquitination Assay
  • PROTAC in Vivo Evaluation
    • PROTAC in animal model
  • Targeted Protein Degradation
    • Destabilization Domains (DDs)
    • Hydrophobic Tag Services
• Protein Production
  • Anaerobic Fermentation
    • Lactic Acid Bacteria Fermentation
  • Antimicrobial Peptides Services
  • Cell Line Development
    • Cell Medium Development and QC Tests
    • Stable Cell Line Construction for Protein Expression
  • Custom Lysate Preparation Service
  • Process and Production
    • Cell Line Development for Industrial Manufacturing
    • cGMP Compliant Protein Production
    • GLP Compliant Protein Production
    • Large-Scale Protein Production
    • Process Development Service
  • Protein Expression
    • Corynebacterium glutamicum Expression Systems
    • Animal-Free Expression
      • Nicotiana Tabacum Expression System
      • Lettuce Transient Expression System
      • Microalgae expression system
    • Bacteria Expression
    • Baculovirus-Mammalian cell Protein Expression System
    • Cell-Free Protein Expression
      • E.coli Cell-Free Expression
      • Insect cell lysate Cell-Free Protein Expression
      • Rabbit Reticulocyte Lysate Cell-Free Protein Expression
      • Tobacco Cell-Free Protein Expression
      • Wheat Germ Extract cell-free expression
    • Fusion Protein Expression
    • Insect Cell Expression
    • Mammalian Cell Expression
    • NovEgg Expression System
    • Protein co-expression
      • Protein Co-Expression Service in E. coli System
      • Protein Co-Expression Service in Mammalian Cells
    • Trimeric Protein Production Service
    • Yeast Expression
    • Antibiotic-free expression system
  • Protein Labeling
    • Incorporation of Bioorthogonal Handles
      • Direct Chemical Modification
      • Incorporation of Unnatural Amino Acids
      • Incorporation of Unnatural Sugars
    • PEGylation Products and Services
      • Custom PEGylation Services
      • PEGylation Products
    • Protein Biotinylation Service
    • Protein Conjugation Service on Beads
    • Protein-Fluorophore Bioconjugation
    • Protein/Peptide Crosslinking
      • Chemical Crosslinking Services
      • Enzymatic Crosslinking Services
      • Photocrosslinking Services
    • Quantum Dot Labeling
  • Protein Mutagenesis
  • Protein Mutant Library Construction
• Bioinformatics Platform Introduction
  • In Silico Protein-protein Interactions Prediction
  • Bioinformatics Data Management
  • Biological Data Analysis
  • Computational Protein Analysis
    • Molecular Dynamics Simulation
    • Protein Structure Modeling
      • Antibody Modeling
      • Fold Recognition
      • Fusion Protein Modeling
      • Homology Modeling
      • Membrane Protein Modeling
      • Post-translational Modification
  • Custom Bioinformatics Software Development
  • Virtual Screening
    • 3D-QSAR
    • ADME/Tox Prediction
    • Hybrid methods
    • Ligand-based virtual screening (LBVS)
    • Molecular Docking
      • Antibody–Antigen Docking
      • Protein–Carbohydrate Docking
      • Protein–Ligand Docking
      • Protein–Lipid Docking
      • Protein–Nucleic Acid Docking
      • Protein–Peptide Docking
      • Protein–Protein Docking
    • Quantum Mechanics/Chemistry in Drug Design
    • Structure-based virtual screening (SBVS)
• Protein Analysis
  • Complement Assays
    • Complement Component Assays
    • Functional Complement Assays
  • Enzyme Activity Assay Service
    • In Vitro Protein Kinase Assay Service
    • Cell-based Kinase Assays
    • CYP Inhibition Screen Assay Using LC-MS/MS
    • Enzyme Activity Assay
    • Enzyme Target & Activator & Inhibitor Screening Service
  • Nucleic Acid-Protein Interactions
    • DNA-Protein Interactions
      • Chromatin Immunoprecipitation (ChIP) Assay Service
      • DNase I Footprinting Assay Service
      • Electrophoretic Mobility Shift Assay (EMSA) Service
      • CUT&RUN-Sequencing Service
      • ChIRP-Seq Service
    • RNA-Protein Interactions
      • CLIP-Seq Service
      • RIP-Seq Service
  • Optogenetics services
  • Plasma Protein Binding Assay
  • Protein Aggregation Analysis
  • Protein Interaction Analysis Services
    • Bio-Layer Interferometry (BLI)
    • Biomembrane Force Probe (BFP)
    • Co-Immunoprecipitation (Co-IP)
    • Enzyme Fragment Complementation Assay (EFCA)
    • Isothermal Titration Calorimetry
    • Membrane Protein Binding Screening and Profiling
    • Protein Binding Site Mapping
    • Proximity-dependent Biotin Identification (BioID) Service
    • Pull Down Assay
    • Surface Plasmon Resonance (SPR) Service
    • Yeast Two-Hybrid Screening
      • Membrane-Based Yeast Two-Hybrid Screening
      • Nucleus-based Yeast Two-Hybrid Screening
  • Screening and Profiling Services
    • GPCR Screening Service
    • Ion Channel Screening Service
    • Transporter Screening Service
  • Transcription Factor Analysis
  • Biomarker Discovery and Development Service
  • Genotoxic Impurities Analysis
• Drug Development
  • Biopharmaceutical Process and Product Related Impurities Analysis
  • Biosimilar Testing Services
  • Immunogenicity Testing Services
  • Induced Protein Degradation
  • PROTAC Discovery and Development
    • E3 Ligase/Target Protein Development
    • Ligand Design and Development
  • Stability Analysis Services

INQUIRY

CONTACT US

•   Email: info@profacgen.com
•   Tel: 1-631-448-8149
•   Fax: 1-631-938-8127
•   45-1 Ramsey Road, Shirley, NY 11967, USA