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• Protein Biotinylation Service • Custom Lysate Preparation Service • Immunogenicity Test Service > Anti-Drug Antibody (ADA) Evaluation Service > Neutralizing Antibody (NAb) Test Service • Anti-Drug Antibody (ADA) Evaluation Service • Neutralizing Antibody (NAb) Test Service • Thanksgiving Day Sale • Enzyme Target & Activator & Inhibitor Screening Service • GPCR Screening Service • Ion Channel Screening Service • Bio-Layer Interferometry (BLI) • Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis > Electrophoretic Mobility Shift Assay (EMSA) Service > Chromatin Immunoprecipitation (ChIP) Assay Service > DNase I Footprinting Assay Service • Transporter Screening Service • Chromatin Immunoprecipitation (ChIP) Assay Service • Enzyme Activity Assay Service • DNase I Footprinting Assay Service • Co-Immunoprecipitation (Co-IP) • Surface Plasmon Resonance (SPR) Service • Pull Down Assay • Protein Mutant Library Construction • C1 Expression System • Protein Conjugation Service on Beads • Bacteria Expression • Yeast Expression • Mammalian Cell Expression • Insect Cell Expression • Animal-Free Expression • Cell-Free Protein Expression • Large-Scale Protein Production • PEGylation Products and Services > Custom PEGylation Services > PEGylation Products • Basic Protein Characterization • Protein Labeling • Stable Cell Line Construction for Protein Expression • Protein Mutagenesis • Protein Interaction Analysis Services > Bio-Layer Interferometry (BLI) > Co-Immunoprecipitation (Co-IP) > Surface Plasmon Resonance (SPR) Service > Pull Down Assay > Yeast Two-Hybrid Screening > Membrane-Based Yeast Two-Hybrid Screening > CLIP-Seq Service > Protein Binding Site Mapping > Isothermal Titration Calorimetry > Membrane Protein Binding Screening and Profiling • Yeast Two-Hybrid Screening • Membrane-Based Yeast Two-Hybrid Screening • Recombinant Protein or Antibody Production • Protein Expression Systems > Nicotiana Tabacum Expression System > Bacterial Expression Systems(E. coli / Bacillus) > Yeast Expression Systems(P pastoris / S cerevisiae) > Baculovirus-insect Cell Expression Systems > Mammalian Expression Systems(CHO / 293) • PEGylation Services • Stable Cell Line Construction for Protein Expression • Process Development • cGMP Manufacturing • QC and QA • Bacterial Manufacturing • CLIP-Seq Service • Protein Binding Site Mapping • Quantum Dot Labeling • Protein Expression > Custom Lysate Preparation Service > C1 Expression System > Protein Co-Expression Service in E. coli System > Protein Co-Expression Service in Mammalian Cells > Trimeric Protein Production Service > Lettuce Transient Expression System > Baculovirus-Mammalian cell Protein Expression System > Fusion protein expression • Protein Co-Expression Service in E. coli System • Protein Co-Expression Service in Mammalian Cells • NovEgg Expression System • ADME/Tox Prediction • Pharmacophore Modeling • Screening and Profiling Services > Enzyme Target & Activator & Inhibitor Screening Service > GPCR Screening Service > Ion Channel Screening Service > Transporter Screening Service • Trimeric Protein Production Service • Incorporation of Bioorthogonal Handles > Incorporation of Unnatural Amino Acids > Incorporation of Unnatural Sugars > Direct Chemical Modification • Incorporation of Unnatural Amino Acids • Incorporation of Unnatural Sugars • Protein/Peptide Crosslinking > Enzymatic Crosslinking Services > Photocrosslinking Services > Chemical Crosslinking Services • Enzymatic Crosslinking Services • Photocrosslinking Services • Chemical Crosslinking Services • Direct Chemical Modification • Protein-Fluorophore Bioconjugation • Custom PEGylation Services • PEGylation Products • Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) • Induced Protein Degradation > Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) > Hydrophobic Tag Services > Destabilization Domains (DDs) • Hydrophobic Tag Services • Destabilization Domains (DDs) • Drug Development • Protein Analysis > Cell-based Kinase Assays > Protein Aggregation Analysis > Complement Component Assays > Functional Complement Assays • Protein Production > Anaerobic Fermentation > Cell Medium Development and QC Tests • Anaerobic Fermentation > Lactic Acid Bacteria Fermentation • Cell-based Kinase Assays • Isothermal Titration Calorimetry • Lactic Acid Bacteria Fermentation • Protein Aggregation Analysis Nicotiana Tabacum Expression System • Lettuce Transient Expression System • Membrane Protein Binding Screening and Profiling • Baculovirus-Mammalian cell Protein Expression System • Complement Component Assays • Functional Complement Assays • Fusion protein expression • Cell Medium Development and QC Tests

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Tel: 1-631-448-8149
Fax: 1-631-938-8127

Photocrosslinking Services

To trigger the crosslinking reaction, photochemistry is one of the most commonly used methods as it offers high efficiency and good orthogonality with biomolecules. Photocrosslinkers, coupled with other analytical methods, can be used to map intracellular networks with high efficiency and great convenience. As one latest progress in bioconjugation techniques, photocrosslinking attracts fast growing attentions and has gained significant shares of the bioconjugation field, especially where conventional crosslinking reactions are not able to deliver the desired result.

Profacgen offers the most up-to-date techniques to ensure successful strategies of photocrosslinking:

  • Applications of latest photocrosslinking technologies;
  • Custom reaction designs for both early research and application development;
  • Highly experienced scientific team;
  • Design, execution, and delivery of non-commercially available reagents

Our selected photocrosslinking reagents include:

  • Diazirine-NHS ester photocrosslinker. The diazirine group can be easily activated with UV light to undergo insertion reactions with proteins.
  • Diazirine-triphenylphosphine photocrosslinker. This reagent provides both bioorthogonal reactivity and photoreactivity for selective crosslinking of azide-containing proteins1.

Diazirine-triphenylphosphine photocrosslinker
Fig1. Diazirine-triphenylphosphine photocrosslinker

  • Benzophenone-based photocrosslinker. Benzophenone derivatives are among the most popular photocrosslinkers due to its good selectivity and compatibility in aqueous environment2.

Benzophenone-based  photocrosslinker
Fig2. Benzophenone-based photocrosslinker

Since photocrosslinking is compatible with native, cellular settings, it is widely used to probe context-dependent interactions. These methods are ideally suited for understanding dynamic cellular activities and for identifying transient interactions. The application of photocrosslinkers has significantly expanded the toolbox for specific problems in protein studies. Profacgen and its renowned collaborators are devoted to assist our customers in finding the best solution for photocrosslinking.

For more information regarding Profacgen’s protein crosslinking service, please contact us or call 1-631-448-8149. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.

References:

  1. Ahad, A. M.; et al. A traceless Staudinger reagent to deliver diazirines. Org. Lett. 2013, 15: 5060-5063.
  2. Hino, N.; et al. Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid. Nat. Meth. 2005, 2: 201-206.
CONTACT US
Email: info@profacgen.com
Tel: 1-631-448-8149
Fax: 1-631-938-8127
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA