To trigger the crosslinking reaction, photochemistry is one of the most commonly used methods as it offers high efficiency and good orthogonality with biomolecules. Photocrosslinkers, coupled with other analytical methods, can be used to map intracellular networks with high efficiency and great convenience. As one latest progress in bioconjugation techniques, photocrosslinking attracts fast growing attentions and has gained significant shares of the bioconjugation field, especially where conventional crosslinking reactions are not able to deliver the desired result.
Profacgen offers the most up-to-date techniques to ensure successful strategies of photocrosslinking:
Our selected photocrosslinking reagents include:
Fig1. Diazirine-triphenylphosphine photocrosslinker
Fig2. Benzophenone-based photocrosslinker
Since photocrosslinking is compatible with native, cellular settings, it is widely used to probe context-dependent interactions. These methods are ideally suited for understanding dynamic cellular activities and for identifying transient interactions. The application of photocrosslinkers has significantly expanded the toolbox for specific problems in protein studies. Profacgen and its renowned collaborators are devoted to assist our customers in finding the best solution for photocrosslinking.
For more information regarding Profacgen’s protein crosslinking service, please contact us or call 1-631-448-8149. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.