Screening Interacting Proteins by Protein Chip

Protein Chip (protein microarray) is a high-throughput protein function analysis technology, which can be used to study protein activity, analyze protein expression profile, study protein-protein interaction, screen protein targeting drugs, etc. The chip consists of a solid phase carrier (such as a nitrocellulose membrane, a bead or a microporous plate) that has been chemically treated with a known protein molecular probe. The capture protein binds to proteins on the support surface and probe molecules, usually labeled with fluorescent dyes or isotopes, are added to the array. Any binding reaction between the probe and the fixed protein emits a fluorescent signal, which is read by a laser scanner and analyzed quickly and automatically with the corresponding computer software.

Figure 1 Examples of different types of protein chips[1].

As shown in the figure, common protein Chips come in the following four ways:

1. Proteins and peptides are immobilized as trapping agents for antibody detection, while anti-immunoglobulin (a common labeled antibody) is used for detection.
2. Protein direct detection mode. Antibodies, sugars or aptamers are fixed and labeled proteins are captured.
3. Sandwich protein detection mode. Detection was performed with labeled secondary antibody.
4. Inverting microarray. Complex samples, such as serum, plasma, or tissue, are fixed in array form and antibodies are used to determine the amount of difference in protein molecules in the screening sample.

Our protein interaction detection service is implemented with high density commercial Chips and has the following comparative advantages:

• Sample size: high throughput, microquantitative.
• Protein Chip: high signal-to-noise ratio, good stability, as far as possible to maintain the natural protein conformation, high specificity.
• Diversity: We detect multiple reactants for each fixed protein on the Chip using fluorescence reporter groups of different wavelengths.
• Relatively high-cost performance ratio.

Purpose of protein Chip:

Workflow of screening interacting proteins by protein chip:

If you are interested in our protein chip service, please feel free to contact us. Profacgen is looking forward to cooperating with you.