SILAC-IP-MS

Stable isotope labeling of amino acids in cell culture (SILAC) with co-immunoprecipitation (Co-IP) to find interacting proteins is an application of quantitative proteomics research. Subsequently, qualitative and quantitative analysis of white was achieved by combining with mass spectrometry label. Therefore, SILAC-IP-MS is also known as the gold standard for studying protein interaction and quantitative analysis of protein interaction.

Figure 1 Summary of the experimental work-flow applied to investigate PRA and PRB interacting partners.

Compared with traditional immunoprecipitation, the protein interactions studied by SILAC-IP-MS have the following characteristics:

• Living cell isotope labeling using SILAC technology is not affected by post-translational protein modifications compared to traditional Dimethyl chemical labeling and can be smoothly quantified.
• Poplar mixing does not need to be carried out after protease cutting and has high repeatability.
• High throughput: the combination of liquid and mass spectrometry maximizes the discovery of interacting proteins.
• Suitable for transmissible cells.

We provide you with a variety of SILAC-IP-MS services, cell lines can be wild-type, overexpressed or gene silenced. The main technical route is as follows: from cell culture, SILAC labeling, protein extraction and purification, heavy and light chain protein mixing, SDS-PAGE, in gel enzymatic hydrolysis, LC-MS detection, and finally we can provide you with mass spectrometry data analysis and related bioinformatics analysis.

SILAC-IP-MS applications are as follows:

• Screening for specific functional proteins
• Protein interaction, modification studies
• Drug target research
• Study on pathogenesis of diseases

Why choose us? What do we have about our SILAC-IP-MS service?

• Full staffing
• Have a professional QC team
• Have a timely technical support and customer service team, can real-time technical communication with customers, tracking services.
• Many years of experience in protein interaction

SILAC-IP-MS service results include:

• Transparent and reliable experimental procedures and associated test parameters, such as western blotting results
• Protein Details
• Mass spectrometry images, bioinformatics analysis results
• The original data

If you want to know more test information and price information, please feel free to contact us and Profacgen will reply to you as soon as possible.