Sign In / Register         Shopping cart (0)

Microscale Thermophoresis (MST)

Microscale Thermophoresis (MST)

Microscale thermophoresis (MST) is a technique for biophysical analysis of biomolecular interactions. MST is based on the detection of temperature induced target fluorescence changes as a function of the concentration of non-fluorescent ligands. MST can be used with almost all kinds of target molecules, if they are fluorescent or can be labeled with fluorescent dyes. The diversity of ligands is greater. The fluorescent target molecule keeps a constant concentration, and the non-fluorescent ligand molecule titrates in a large concentration range.

  • The binding of any type of biomolecule can be studied, including small molecule, multi protein complex, DNA, liposome, nanoparticles, etc
  • Fast delivery time for analytical development and affinity determination
  • Deliverables: dissociation constant KD (binding affinity)

MST is carried out in a thin capillary in free solution, thus providing near natural conditions (no immobilization in any buffer, even in complex biological liquids) and maintenance free instruments. In MST experiment, infrared laser can cause micro temperature gradient, and detect tric and thermophoresis. Tric depends on the microenvironment of the fluorophore and usually changes during binding events. The movement of molecules in a temperature gradient, thermophoresis, depends on three parameters that usually vary with the interaction. Therefore, the dose-response curve can be obtained by plotting the whole MST signal relative to the ligand concentration, from which the binding affinity can be deduced.

Microscale thermophoresis (MST)
Figure 1. The principle of MST technique. (Wikipedia)

MST can be used as following:

  • Single dose screen. MST will tell you whether the target and ligand usually interact through a single dose experiment.
  • Steady state affinity. MST will tell you the exact and true steady-state affinity of the interaction (dissociation constant k d).
  • Protein aggregation. MST will tell you whether the protein sample is aggregated or whether the aggregation is induced by ligand binding.
  • Ligand competition. MST will tell you the competitive effect of multiple adhesives targeted.

Profacgen uses microscale thermoporesis technology to provide a variety of services. You can combine Profacgen services to solve all problems in drug discovery, antibody development, protein biophysics and analysis, and aptamer characterization.

For more information regarding Profacgen’s microscale thermoporesis service, please contact us. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.

Online Inquiry

Fill out this form and one of our experts will respond to you within one business day.