Profacgen provides stable cell line development service for industrial protein manufacturing. We offer various cell line development options, which allow the maximum flexibility in reaching our client’s expectations and accelerate the preclinical drug development process.
Stable expression line construction is recommended for reproducible and cost-effective protein production, when large amount of recombinant protein is required. Profacgen guarantees well-developed stable cell lines with robust and reproducible production of the target protein.
Stable cell line development is significant for:
|Consistent high level expression of the protein of interest|
|Cell line development in serum free media to meet regulatory requirements|
|Expression of large functionally active multimeric proteins due to the presence of complex post-translation modifications (i.e., glycosylation and disulphide bond linkage)|
|Ease of transfer of cell lines for cGMP compliant production|
At Profacgen, we use lentiviral vector for efficient gene delivery and to achieve high integration rate. Limited dilution, antibiotic screening or flow cytometry will be then applied to screen for positive clones. We can also generate Tet-ON inducible stable cell lines and use Doxycycline to induce target gene expression.
State-of-the art screening strategies to enable better decisions on cell lines with high yield and reproducibility to progress
Establishment of high performing basal and feed media strategies for diverse cell lines
Product quality optimization and control during process development, and scale up to manufacturing
Streamline manufacturing operations and provide advantages in technology transfer via elegant process design.
Feel free to contact us for more details of our Cell Line Development service. Our experts will provide and help design an optimal solution for your project and trouble-shoot for you throughout the whole process.
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