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Surface plasmon resonance (SPR) Service


Profacgen provides a comprehensive panel of services for the study of protein-protein interactions, of which the Surface Plasmon Resonance (SPR) analysis is commonly used by our customers for the highly accurate and sensitive kinetics and affinity measurement. For SPR analysis, the ligand-of-interest is immobilized on the surface of the biosensor chip, while the analyte is injected to flow through the chip surface. Dynamic change of the SPR angle can be detected and assayed for binding affinity when interaction happens between the ligand and the analyte.

The SPR technique is based on a complex optical phenomenon. When photon reaches the interface of two medium with different refractive indexes at a specific angle range, the resonant oscillation of surface plasmon generated by metal free electron can be induced. The resonance also requires that the frequency of incident photon matches the natural oscillating frequency of the surface plasmon. Once the resonance happens, the energy of the reflected photon will be absorbed and the light dramatically weakened. Within the SPR angle range, the SPR angle (or resonance angle) is defined as the angle at which nearly no reflect photon can be detected. Since the introduction of SPR into the immune sensor field by Liedberg, Nylander and Lundström in 1983, surface plasmon optical biosensor has been extensively studied and widely used. Later on, the SPR technology was further developed to study the interaction between ligand and analyte on a biosensor chip. As the SPR angle changes with surface refractive indexes, which is in direct proportion to the molecular mass of the biomolecule attached to the metal surface, the binding of the analyte can be detected at high sensitivity.

Principle of the SPR technology [1]

Principle of the SPR technology

Profacgen provides professional SPR analysis service. Our service has been extensively applied to determining the interaction between proteins, peptides, oligonucleotides, bacteria, cells and small molecular compounds.

Advantages of the SPR technology:

  Sample requires no labeling or modification before analysis
  Low requirement on sample purity
  In situ, real time dynamics study under natural conditions
  High throughput and high sensitivity analysis
  Ease of manipulation
  Requires only small amount of sample

Our strengths:

  Advanced equipment and technique

  Experienced scientific team

  Highly reliable and reproducible result

  Short turnaround time

  Best price in the market

Please feel free contact us for more information of our SPR service.

See the instruction of surface plasmon resonance (SPR) service. SPR technology has broad application in physics, chemistry and biology. Profacgen provides various types of SPR biosensors for various applications. The sample from costumer for SPR should meet several requirements. Please see detail in the instruction. And our experts will design the experiment according to your specific requirements.
Click here to download the Service Instruction.

References:

[1] Chang L, Tingjun L, Kosuke I, et al. Real-time monitoring biomarker expression of carcinoma cells by surface plasmon resonance biosensors[J]. Chem Commun, 2012, 48(84): 10389-91.

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