Service Search Product Search Gene Search
HOME

SERVICES

• Protein Biotinylation Service • Custom Lysate Preparation Service • Immunogenicity Test Service > Anti-Drug Antibody (ADA) Evaluation Service > Neutralizing Antibody (NAb) Test Service • Anti-Drug Antibody (ADA) Evaluation Service • Neutralizing Antibody (NAb) Test Service • Thanksgiving Day Sale • Enzyme Target & Activator & Inhibitor Screening Service • GPCR Screening Service • Ion Channel Screening Service • Bio-Layer Interferometry (BLI) • Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis > Electrophoretic Mobility Shift Assay (EMSA) Service > Chromatin Immunoprecipitation (ChIP) Assay Service > DNase I Footprinting Assay Service • Transporter Screening Service • Chromatin Immunoprecipitation (ChIP) Assay Service • Enzyme Activity Assay Service • DNase I Footprinting Assay Service • Co-Immunoprecipitation (Co-IP) • Surface Plasmon Resonance (SPR) Service • Pull Down Assay • Protein Mutant Library Construction • C1 Expression System • Protein Conjugation Service on Beads • Bacteria Expression • Yeast Expression • Mammalian Cell Expression • Insect Cell Expression • Animal-Free Expression • Cell-Free Protein Expression • Large-Scale Protein Production • PEGylation Products and Services > Custom PEGylation Services > PEGylation Products • Basic Protein Characterization • Protein Labeling • Stable Cell Line Construction for Protein Expression • Protein Mutagenesis • Protein Interaction Analysis Services > Bio-Layer Interferometry (BLI) > Co-Immunoprecipitation (Co-IP) > Surface Plasmon Resonance (SPR) Service > Pull Down Assay > Yeast Two-Hybrid Screening > Membrane-Based Yeast Two-Hybrid Screening > CLIP-Seq Service > Protein Binding Site Mapping > Isothermal Titration Calorimetry > Membrane Protein Binding Screening and Profiling • Yeast Two-Hybrid Screening • Membrane-Based Yeast Two-Hybrid Screening • Recombinant Protein or Antibody Production • Protein Expression Systems > Nicotiana Tabacum Expression System > Bacterial Expression Systems(E. coli / Bacillus) > Yeast Expression Systems(P pastoris / S cerevisiae) > Baculovirus-insect Cell Expression Systems > Mammalian Expression Systems(CHO / 293) • PEGylation Services • Stable Cell Line Construction for Protein Expression • Process Development • cGMP Manufacturing • QC and QA • Bacterial Manufacturing • CLIP-Seq Service • Protein Binding Site Mapping • Quantum Dot Labeling • Protein Expression > Custom Lysate Preparation Service > C1 Expression System > Protein Co-Expression Service in E. coli System > Protein Co-Expression Service in Mammalian Cells > Trimeric Protein Production Service > Lettuce Transient Expression System • Protein Co-Expression Service in E. coli System • Protein Co-Expression Service in Mammalian Cells • NovEgg Expression System • ADME/Tox Prediction • Pharmacophore Modeling • Screening and Profiling Services > Enzyme Target & Activator & Inhibitor Screening Service > GPCR Screening Service > Ion Channel Screening Service > Transporter Screening Service • Trimeric Protein Production Service • Incorporation of Bioorthogonal Handles > Incorporation of Unnatural Amino Acids > Incorporation of Unnatural Sugars > Direct Chemical Modification • Incorporation of Unnatural Amino Acids • Incorporation of Unnatural Sugars • Protein/Peptide Crosslinking > Enzymatic Crosslinking Services > Photocrosslinking Services > Chemical Crosslinking Services • Enzymatic Crosslinking Services • Photocrosslinking Services • Chemical Crosslinking Services • Direct Chemical Modification • Protein-Fluorophore Bioconjugation • Custom PEGylation Services • PEGylation Products • Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) • Induced Protein Degradation > Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) > Hydrophobic Tag Services > Destabilization Domains (DDs) • Hydrophobic Tag Services • Destabilization Domains (DDs) • Drug Development • Protein Analysis > Cell-based Kinase Assays > Protein Aggregation Analysis • Protein Production > Anaerobic Fermentation • Anaerobic Fermentation > Lactic Acid Bacteria Fermentation • Cell-based Kinase Assays • Isothermal Titration Calorimetry • Lactic Acid Bacteria Fermentation • Protein Aggregation Analysis Nicotiana Tabacum Expression System • Lettuce Transient Expression System • Membrane Protein Binding Screening and Profiling

CONTACT US

Tel: 1-631-448-8149
Fax: 1-631-938-8127

Chemical Crosslinking Services

Crosslinkers are widely used in modifying nuclei acids, drugs and polymers. The same principle applies to protein modification and labeling. Based on factors such as reactivities and spacer length, the reagents for crosslinking are classified into different types, each having its own specific function and application. In particular, introducing protein-protein crosslinks using bifuncational small molecules with highly selective reactivities enables specific conjugations to amines, thiols, carbohydrate and other functional groups. Our team of well-trained biological and organic chemists assist customers in a way precisely adapted to their requirements, serving either specific research goals or as a partnership for a full range support.

Profacgen currently provide a majority of chemical crosslinking services (custom reagents and services available upon request):

chemical crosslinking-1

Amine to amine crosslinking. Amine-specific crosslinker

Thiol to thiol crosslinking. Crosslinking reagents utilize maleimide and pyridyldithiol derivatives to conjugate thiols from protein and peptides in a selective manner. Also available is the PEG containing crosslinkers providing enhanced solubility and reduced immunogenicity.

Thiol to amine crosslinking. When conjugation is needed between two different groups, heterobifunctional reagents are used instead. We offer NHS-maleimide, NHS-pyridyldithiol and NHS-haloacetyl crosslinkers.

Thiol/amine to carbohydrate. Heterobifunctional reagents bearing maleimide/NHS ester and hydrazine groups selectively crosslink thiols/amines from proteins to aldehydes from carbohydrates.

Examples of popular chemical crosslinking reagents

 NHS ester crosslinker
 NHS-maleimide-crosslinker.png
  maleimide-hydrazine crosslinker
 bismaleimidoethane

                             

The interaction between proteins are often hard to detect and define. Introduction of selective, stable chemical linkage is able to establish permanent relationships between molecules that would otherwise be transiently associated. The ease and reliability of using crosslinking strategies have greatly assisted researchers in protein study and engineering over the decades. Herein, with our broad expertise and technologies in-house, our priority is to deliver contract research and project development with premium quality and to make added value for our customers.

Hundreds of customers have endorsed the advantages of choosing Profacgent:

  • Highly customized service to meet specific needs
  • Large selection of crosslinking reagents
  • Interdisciplinary research team with well-experienced technicians
  • Full support from ideas, through process trouble-shooting, to delivery
  • State-of-the-art facilities
  • 24H-available customer service

For more information regarding Profacgen’s protein crosslinking service, please contact us or call 1-631-448-8149. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.

CONTACT US
Email: info@profacgen.com
Tel: 1-631-448-8149
Fax: 1-631-938-8127
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA