Proteins and protein derivatives are prone to aggregation under certain circumstances, e.g., sudden pH or temperature changes. Such aggregation may have detrimental effects, for example, aggregation of therapeutic proteins can lead to unexpected side effect or even fatal immune response. Despite of the importance of protein aggregates as indicator of stability, they are sometimes tough to measure, and often require advanced analytical instruments for quality measurement.
As an industry-leader in bioanalysis, Profacgen has developed a comprehensive platform for protein aggregation analysis. Our routinely used systems and methods offer a combined toolbox of well-established conventional approaches that are excellent in reliability along with some of the newly developed strategies that deliver unprecedented high resolution and efficacy.
Size-exclusion chromatography (SEC)
SEC is designed for high-resolution separation of various proteins and protein products, including monomers, aggregates and Fc/Fab fragments of monoclonal antibodies. The SEC columns are chemically inert and compatible with a wide range of experimental conditions, providing superior performance at relatively low cost.
Hydrophobic interaction chromatography (HIC)
Proteins in their native state exhibit different hydrophobicity as they differ in chemical composition. HIC takes advantage of hydrophobic interactions to determine aggregation of protein samples. Our selection of HIC columns offer improved biocompatibility and sensitivity compared to most products on the market.
Dynamic light scattering (DLS)
DLS is most commonly used to measure proteins and nanoparticles with sizes ranging from microns to nanometers. The size of a particle can be determined by monitoring the random changes of the scattered light from a solution where the particles are dispersed in. When aggregation occurs, the enlarged particle size will lead to a change in measurement – the hydrodynamic radius becomes larger.
Image-based particle analysis
Unlike the abovementioned methods, image-based analysis allows one to quantifiable morphological parameters to the analysis. The sample data can be analyzed automatically using computer programs, and also tends to be more robust and reproducible. Images with high resolution directly provides an accurate picture of protein aggregation pattern.
Profacgen is a trusted partner for your next protein analysis project. Together with our internationally renowned collaborators, we are proud to offer the most up-to-date analytical technologies and instrumentation. We believe that every project deserves its attention, so a skilled team of scientists will work closely with our clients to ensure the delivery of quality services that meet every specific need of the client. For more information, please contact us.