Schematic Diagram of ChIP-Seq 
Chromatin immunoprecipitation (ChIP) assay is a powerful method for the investigation of DNA-protein interaction. It can reflect the physiological binding status between proteins and chromosomal DNA based on the in vivo technique design and specificity of antigen-antibody interaction. Profacgen provides ChIP assay service with the combination of our Next Generation Sequencing (NGS) technology to map DNA binding sites for your protein of interest on a genome-wide scale.
ChIP is commonly used in the study of transcriptional factor binding site or protein modification site. With the development of NGS, the combination of CHIP and NGS, Chromatin Immunoprecipitation Sequencing (ChIP-Seq), is now able to screen and identify the protein binding sites on the entire chromosomal scale with high efficiency and accuracy. Briefly, in vivo protein-DNA interaction complex is fixed and digested into small fragments and then immunoprecipitated by an antibody against the DNA-binding protein. This way, the protein-binding DNA fragments are enriched and can be analyzed by NGS so the protein binding sites in the entire genome can be mapped and characterized. ChIP-Seq is a valuable tool for discerning and quantifying the specific DNA sequences where proteins bind or epigenetic modifications exist.
Profacgen is a professional ChIP service provider that offers comprehensive ChIP solutions. You can simply provide us with your cell or tissue sample. We provide service from protein-DNA complex fixation, chromatin digestion, protein-DNA complex immunoprecipitation, high-throughput sequencing to data analysis. Our optimized reagents and protocol guarantees minimal background with increased sensitivity and specificity. Moreover, QC is included in each critical step to ensure accuracy.
Professional bioinformatics analysis
High resolution and high signal-to-noise ratio results
Best price in market
Please feel free to contact us for details about our professional ChIP service. Our team will provide customized experimental design for your project based on your specific requirements.
 Park P J. ChIP-Seq: advantages and challenges of a maturing technology[J]. Nat Rev Genet, 2009, 10(10): 669-80.