Lactose Content Assay Kit (CASQL-K029S)

Cat.No.

CASQL-K029S

Method

Spectrophotometry

Product size

24T

Product description

Lactose, under the action of β-galactosidase, is broken down into galactose and glucose. Glucose is then oxidized by a specific enzyme to produce a (pink) red product that reacts with the color reagent, which has a maximum absorption peak at 510nm. The lactose content is obtained by correcting the background value of free glucose.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvette (path length 1cm), balance, pipette, mortar and pestle, centrifuge, distilled water.