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Methylglyoxal (methylglyoxal, MG), also known as pyruvaldehyde, is a byproduct of several metabolic pathways and a common toxic aldehyde compound produced by plants under environmental stress. High concentrations of MG are cytotoxic, while low concentrations act as a signaling molecule, regulating various physiological processes such as cell metabolism, seed germination, plant growth, development, reproduction, and the acquisition of stress resistance, thus MG has a dual role. The product of methylglyoxal (MG) and 1,2-phenylenediamine has a maximum absorption peak at 336nm, and the content of methylglyoxal (MG) in the sample can be calculated by detecting the value at 336nm.