Recombinant Human EGFR Protein(Avi&His tag), Biotinylated (HH0042EL)

Cat.No.

HH0042EL

Synonyms

EGFR; ERBB1; ERBB; HER1; mENA; PIG61;

Species

Human

Accession

EGFR

Source

HEK293

Tag

Avi & His

Form

Lyophilized from sterile PBS, pH7.4, 10% Trehalose.

Bio-activity

Immobilized Human EGF, Tag Free at 5 μg/mL (100 μl/well) can bind Biotinylated Human EGF R with a linear range of 0.6-10 μg/mL.
Measured by its ability to bind Human EGF, Tag Free in the SPR assay (Biacore 2000) with an estimated KD of 120nM.
Immobilized

Molecular Mass

Biotinylated recombinant Human EGFR Protein has a calculated MW of 71.2 kDa. The protein migrates as 110-115 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.