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Insect cell lysate Cell-Free Protein Expression

Insect cell lysate Cell-Free Protein Expression

Cell-free protein expression continuous exchange system based on insect cell extracts is a new in vitro cell-free expression system capable of synthesizing complex proteins such as membrane proteins.

Profacgen provides an insect lysate cell-free protein expression system with a membrane protein and glycosylation environment. The lysate contains some endogenous microsomal vesicles originating from the endoplasmic reticulum,which can be used as a place where the target protein is fused with a signal peptide and then modified.

Protocal of the insect lysate cell-free protein expression system:

  1. Preparation of Spodoptera frugiperda (Sf21) cell extracts;
  2. Add amino acid, T7 RNA polymerase, and energy substance to form the reaction system; or you can use our TNT system which is a single-tube, coupled transcription/translation system for the cell-free expression of proteins from DNA templates;
  3. Add plasmid DNA or PCR products, synthesize mRNA in vitro under the action of RNA polymerase, incubate and detect; you can also use our companion vectors to improve translation efficiency; proteins are expressed from genes cloned downstream of the T7 promoter;
  4. The mRNA released after translation is recycled using a cell-free system to synthesize the protein, and the mRNA is inactivated after repeated several times.

Advantages of the insect lysate cell-free protein expression system:

  1. Easy cell breakage and quick preparation of extract
  2. Various eukaryotic-specific modificationskede
  3. Signal sequence processing
  4. High-yield protein available for many downstream experiments

Profacgen's cell-free expression platform can provide you with comprehensive technical services, specializing in the special properties of the target protein, and professionally solve protein expression related problems, such as low protein production, expression of special proteins (such as membrane proteins, toxic proteins, etc.), and preparation of proteins Complex, parallel synthesis of many different proteins, etc. We always put your needs first, and if you have any questions about the results, we can help you verify. Profacgen is your ideal partner. If you are interested in our services, please feel free to contact us for more information.

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