Mammalian cell expression systems are the best choice for the production of eukaryotic proteins, especially when correct folding and post-translational modification (glycosylation, phosphorylation, etc.) is required. They produce eukaryotic recombinant proteins in the most natural state, with native tertiary structure, physiochemical characteristics, and bioactivities. They have been successfully applied in the biopharmaceutical production of cytokines, monoclonal antibodies, growth factors and so on. The most widely-used mammalian cell lines are HEK293 and CHO cells.
Human Embryonal Kidney 293 (HEK293) cells have been extensively used for the transient expression of recombinant proteins. Their ease of culture and highly efficient transfection rate make them especially suitable for customized protein production for research use.
Human Embryonic Kidney 293 (HEK293) cells are a specific cell line originally deerved from human embryonic kidney cells grown in tissue culture.
They are very easy to grow and transfect very readily, thus widely used in cell biology research and biotechnology industry.
Chinese Hamster Ovary (CHO) cells, which have been successfully used for the manufacture of FDA-approved therapeutic proteins and antibodies, are prominent in stable expression. Stably transfected CHO cell line is the most suitable mammalian host for high-yield production of recombinant proteins. They are adaptable to diverse culture conditions. Protein purification from CHO culture is also relatively easy because of their secretory feature.
Chinese hamster ovary(CHO) cells are a cell line derived from the ovary of the chinese hamster.
They’re often used in biological and medical research.
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