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• Protein Biotinylation Service • Custom Lysate Preparation Service • Immunogenicity Test Service > Anti-Drug Antibody (ADA) Evaluation Service > Neutralizing Antibody (NAb) Test Service • Anti-Drug Antibody (ADA) Evaluation Service • Neutralizing Antibody (NAb) Test Service • Thanksgiving Day Sale • Enzyme Target & Activator & Inhibitor Screening Service • GPCR Screening Service • Ion Channel Screening Service • Bio-layer Interferometry (BLI) Service • Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis > Electrophoretic Mobility Shift Assay (EMSA) Service > Chromatin Immunoprecipitation (ChIP) Assay Service > DNase I Footprinting Assay Service • Transporter Screening Service • Chromatin Immunoprecipitation (ChIP) Assay Service • Enzyme Activity Assay Service • DNase I Footprinting Assay Service • Co-Immunoprecipitation (Co-IP) • Surface plasmon resonance (SPR) Service • Pull Down Assay • Protein Mutant Library Construction • C1 Expression System • Protein Conjugation Service on Beads • Bacteria Expression • Yeast Expression • Mammalian Cell Expression • Insect Cell Expression • Animal-free Expression • Cell-free protein expression • Large-scale Protein Production • PEGylation Products and Services > Custom PEGylation Services > PEGylation Products • Basic Protein Characterization • Protein Labeling • Stable Cell Line Construction for Protein Expression • Protein Mutagenesis • Protein Interaction Analysis Services > Bio-layer Interferometry (BLI) Service > Co-Immunoprecipitation (Co-IP) > Surface plasmon resonance (SPR) Service > Pull Down Assay > Yeast Two-Hybrid Screening > Membrane-based Yeast Two-Hybrid Screening > CLIP-Seq Service > Protein Binding Site Mapping > Isothermal Titration Calorimetry • Yeast Two-Hybrid Screening • Membrane-based Yeast Two-Hybrid Screening • Recombinant Protein or Antibody Production • Protein Expression Systems > Nicotiana tabacum Expression System > Bacterial Expression Systems(E. coli / Bacillus) > Yeast Expression Systems(P pastoris / S cerevisiae) > Baculovirus-insect Cell Expression Systems > Mammalian Expression Systems(CHO / 293) • PEGylation Services • Stable Cell Line Construction for Protein Expression • Process Development • cGMP Manufacturing • QC and QA • Bacterial Manufacturing • CLIP-Seq Service • Protein Binding Site Mapping • Quantum Dot Labeling • Protein Expression > Custom Lysate Preparation Service > C1 Expression System > Protein Co-expression Service in E. coli System > Protein Co-expression Service in Mammalian Cells > Trimeric Protein Production Service > Lettuce Transient Expression System • Protein Co-expression Service in E. coli System • Protein Co-expression Service in Mammalian Cells • NovEgg Expression System • ADME/Tox Prediction • Pharmacophore Modeling • Screening and Profiling Services > Enzyme Target & Activator & Inhibitor Screening Service > GPCR Screening Service > Ion Channel Screening Service > Transporter Screening Service • Trimeric Protein Production Service • Incorporation of Bioorthogonal Handles > Incorporation of Unnatural Amino Acids > Incorporation of Unnatural Sugars > Direct Chemical Modification • Incorporation of Unnatural Amino Acids • Incorporation of Unnatural Sugars • Protein/peptide Crosslinking > Enzymatic Crosslinking Services > Photocrosslinking Services > Chemical Crosslinking Services • Enzymatic Crosslinking Services • Photocrosslinking Services • Chemical Crosslinking Services • Direct Chemical Modification • Protein-fluorophore Bioconjugation • Custom PEGylation Services • PEGylation Products • Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) • Induced Protein Degradation > Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) > Hydrophobic Tag Services > Destabilization Domains (DDs) • Hydrophobic Tag Services • Destabilization Domains (DDs) • Drug Development • Protein Analysis > Cell-based Kinase Assays > Protein Aggregation Analysis • Protein Production > Anaerobic Fermentation • Anaerobic Fermentation > Lactic Acid Bacteria Fermentation • Cell-based Kinase Assays • Isothermal Titration Calorimetry • Lactic Acid Bacteria Fermentation • Protein Aggregation Analysis Nicotiana tabacum Expression System • Lettuce Transient Expression System

CONTACT US

Tel: 1-631-448-8149
Fax: 1-631-938-8127

Large-scale Protein Production

Large-scale recombinant protein production is becoming increasingly important for applications in the field of proteomics, structural biology and protein therapeutics. When large quantities of protein (from mg to g amounts) needs to be produced from either bacterial (e.g. Escherichia coli) or eukaryotic cell-based culture (e.g. yeast, insect and mammalian cell), cells must be cultured in large volume by the implementation of fermentation techniques. Fermentation can improve efficiency by increasing cell density and provide easy-to-handle large culture over 4 liters. Depending on the high cell density as well as fermentation strategy, we can optimize cell culture conditions to increase cell mass and finally improve recombinant protein yield.

Profacgen is fully equipped to meet this production demand for large quantities of pure and active protein. Our versatile protein expression platform is equipped with a comprehensive series of bioreactors (from fermenters to wave mixers), which can accommodate protein production projects at different scales, ranging from milligrams to hundreds of grams.

Capabilities and Specifications of Different Expression Systems

Expression System Capabilities Features
Bacteria Fermentation culture up to 2000 L
  1. i. Homogeneous recombinant protein production with purity over 98% for crystallization.
  2. ii. Protein yield up to several grams.
Yeast Fermentation culture up to 2000 L
  1. i. Homogeneous recombinant protein production with purity over 98% for crystallization.
  2. ii. Protein yield up to grams.
Insect/Baculovirus Culture up to 2000 L
  1. iii. Homogeneous recombinant protein production with purity over 98% for crystallization.
  2. iv. High titer recombinant Baculovirus stock.
  3. v. Protein yield up to hundreds of milligrams.
Mammalian Cells Stable cell line culture up to 500 L
  1. i. Protein yield up to milligrams.
  2. ii. Allow transient expression for the production of up to 10 mg protein.

From Bench-Top Fermenter to Bioprocess Scale-Up Facility

From Bench-Top Fermenter to Bioprocess Scale-Up Facility

Improvements for Upstream and Downstream Technologies

Creation of Highly Productive Cell Lines   Modified gene vectors combined with optimized transfection and screening methods
  Engineered cell lines also improve protein characteristics
  Select highly productive clones
Fermentation Optimization   Improve media and feeding strategies
  Optimize growth and physicochemical conditions

Profacgen provides you with an ultimate solution for large-scale protein production. Our service can be tailored according to your specific requirements. Our expertise guarantees high protein quality, short turnaround time, and best price in the market!

For more information or project quotations, you are welcome to contact us at 1-631-559-9269, or send an email to .

CONTACT US
Email: info@profacgen.com
Tel: 1-631-448-8149
Fax: 1-631-938-8127
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA