Multi-protein assemblies can be found in a wide range of biological processes such as transcription, translation, RNA splicing, signaling cascades, protein transport, trafficking and degradation. After realizing that proteins do not work solo but interact extensively with one another as stable or transient complexes, life science researchers have directed their attentions to multi-protein-complexes for structural and functional studies.
Protein complex development is also important in the biotech industry, metabolic engineering, and even bio-pharm therapy area. Reconstruction of multi-proteins by co-expression has been proven a powerful strategy that provides a rapid and efficient way to produce complexes in soluble form as well as to investigate protein-protein interaction network.
As most protein complexes under investigation is from the mammalian system, mammalian cells, therefore, become the most ideal host, which provides the most native-like conditions for ectopically expressed mammalian genes. With extensive expertise in protein manufacture, Profacgen provides various strategies to produce recombinant protein complexes in mammalian cells, such as transient co-transfection, viral infection and stable integration of expression constructs into the host’s genome. Transient co-transfection of multiple, separate plasmids carrying individual genes represents the most frequently and successfully employed technique for functional studies.
Available cell lines:
Protein co-expression technology has been used in:
Profacgen provides several approaches for protein co-expression service. The easiest is to have multiple genes encoded by one vector or virus and co-transform or co-infect the host with several of them. Alternatively, each gene is encoded by a single vector or virus, either under the control of a single promoter or of several promoters, and a single transformation or infection is required. Profacgen offers unprecedented expertise and high quality custom co-expression service with competitive pricing. Request more information to see how Profacgen could be assistance.
1. Or Matalon, Amnon Horovitz and Emmanuel DLevy. Different subunits belonging to the same protein complex often exhibit discordant expression levels and evolutionary properties. Current Opinion in Structural Biology. Volume 26, June 2014, Pages 113-120.
2. Zahra Assur, Wayne A. Hendrickson and Filippo Mancia. Tools for Co-expressing Multiple Proteins in Mammalian Cells. Methods Mol Biol. 2012; 801: 173–187. doi: 10.1007/978-1-61779-352-3 12.