Service Search Product Search Gene Search
HOME

SERVICES

• Protein Biotinylation Service • Custom Lysate Preparation Service • Immunogenicity Test Service > Anti-Drug Antibody (ADA) Evaluation Service > Neutralizing Antibody (NAb) Test Service • Anti-Drug Antibody (ADA) Evaluation Service • Neutralizing Antibody (NAb) Test Service • Thanksgiving Day Sale • Enzyme Target & Activator & Inhibitor Screening Service • GPCR Screening Service • Ion Channel Screening Service • Bio-layer Interferometry (BLI) Service • Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis > Electrophoretic Mobility Shift Assay (EMSA) Service > Chromatin Immunoprecipitation (ChIP) Assay Service > DNase I Footprinting Assay Service • Transporter Screening Service • Chromatin Immunoprecipitation (ChIP) Assay Service • Enzyme Activity Assay Service • DNase I Footprinting Assay Service • Co-Immunoprecipitation (Co-IP) • Surface plasmon resonance (SPR) Service • Pull Down Assay • Protein Mutant Library Construction • C1 Expression System • Protein Conjugation Service on Beads • Bacteria Expression • Yeast Expression • Mammalian Cell Expression • Insect Cell Expression • Animal-free Expression • Cell-free protein expression • Large-scale Protein Production • PEGylation Products and Services > Custom PEGylation Services > PEGylation Products • Basic Protein Characterization • Protein Labeling • Stable Cell Line Construction for Protein Expression • Protein Mutagenesis • Protein Interaction Analysis Services > Bio-layer Interferometry (BLI) Service > Co-Immunoprecipitation (Co-IP) > Surface plasmon resonance (SPR) Service > Pull Down Assay > Yeast Two-Hybrid Screening > Membrane-based Yeast Two-Hybrid Screening > CLIP-Seq Service > Protein Binding Site Mapping > Isothermal Titration Calorimetry • Yeast Two-Hybrid Screening • Membrane-based Yeast Two-Hybrid Screening • Recombinant Protein or Antibody Production • Protein Expression Systems > Nicotiana tabacum Expression System > Bacterial Expression Systems(E. coli / Bacillus) > Yeast Expression Systems(P pastoris / S cerevisiae) > Baculovirus-insect Cell Expression Systems > Mammalian Expression Systems(CHO / 293) • PEGylation Services • Stable Cell Line Construction for Protein Expression • Process Development • cGMP Manufacturing • QC and QA • Bacterial Manufacturing • CLIP-Seq Service • Protein Binding Site Mapping • Quantum Dot Labeling • Protein Expression > Custom Lysate Preparation Service > C1 Expression System > Protein Co-expression Service in E. coli System > Protein Co-expression Service in Mammalian Cells > Trimeric Protein Production Service > Lettuce Transient Expression System • Protein Co-expression Service in E. coli System • Protein Co-expression Service in Mammalian Cells • NovEgg Expression System • ADME/Tox Prediction • Pharmacophore Modeling • Screening and Profiling Services > Enzyme Target & Activator & Inhibitor Screening Service > GPCR Screening Service > Ion Channel Screening Service > Transporter Screening Service • Trimeric Protein Production Service • Incorporation of Bioorthogonal Handles > Incorporation of Unnatural Amino Acids > Incorporation of Unnatural Sugars > Direct Chemical Modification • Incorporation of Unnatural Amino Acids • Incorporation of Unnatural Sugars • Protein/peptide Crosslinking > Enzymatic Crosslinking Services > Photocrosslinking Services > Chemical Crosslinking Services • Enzymatic Crosslinking Services • Photocrosslinking Services • Chemical Crosslinking Services • Direct Chemical Modification • Protein-fluorophore Bioconjugation • Custom PEGylation Services • PEGylation Products • Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) • Induced Protein Degradation > Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) > Hydrophobic Tag Services > Destabilization Domains (DDs) • Hydrophobic Tag Services • Destabilization Domains (DDs) • Drug Development • Protein Analysis > Cell-based Kinase Assays > Protein Aggregation Analysis • Protein Production > Anaerobic Fermentation • Anaerobic Fermentation > Lactic Acid Bacteria Fermentation • Cell-based Kinase Assays • Isothermal Titration Calorimetry • Lactic Acid Bacteria Fermentation • Protein Aggregation Analysis Nicotiana tabacum Expression System • Lettuce Transient Expression System

CONTACT US

Tel: 1-631-448-8149
Fax: 1-631-938-8127

Protein Co-expression Service in E. coli System

Multi-protein complexes are involved in essentially all cellular processes. A protein’s function relies on not only its own physio-chemical properties, but also the interaction status with its partners, and the characteristics of them. Due to the absence of cofactors or post-translational modifications, heterologous protein expression in prokaryote sometimes leads to the yield of insoluble and nonfunctional products. Many protein complexes require co-expression with their native partners in the host cells for proper folding, stability, and activity. Protein co-expression has led to the production of a variety of biological active complexes in sufficient quantities for biochemical, biophysical, structural studies, and high throughput screens.

Protein Co-expression Service in E. coli System

Profacgen has investigated the use of E. coli co-expression techniques in protein manufacturing, and tried to extend from the common binary expression to the more complicated multi-expression. Results from various case studies have indicated co-expression in E. coli host to be a good alternative compared to endogenous purification or in vitro reconstruction from individually expressed components. It is also proved that co-expression of multiple proteins/complexes a useful alternative to the complicated and expensive expression systems, such as yeast, baculovirus or mammalian cell culture.

  • Co-expression of proteins that form a complex
  • Co-expression of subunits of a di- or multimeric protein
  • Co-expression of protein with chaperones and/or foldases

Different strategies of co-expression services provided by Profacgen:

  • Co-expression from one vector: It has the advantage that the primary target and the refolding factor can be carried on a single plasmid.
    • Single vector, single RNA transcript
    • Single vector, multiple RNA transcripts
  • Co-expression from different vectors: To ensure plasmid stability, the vectors should have:
    • Different selectable markers, usually antibiotic resistance markers
    • Different tags
    • Different origins of replication
  • Combination of the two above mentioned methods

In addition, Profacgen can also assist the scientists with protein co-expression network analysis, a powerful approach to identify the tissues and the conditions in which important interactions occur. By integrating the co-expression data with a protein interaction network, the scientists will be able to determine the cellular conditions and tissue specificity of protein interaction network of human proteins.

Reference

M. Cristina Vega. Advanced Technologies for Protein Complex Production and Characterization. ISBN: 978-3-319-27214-6. Edition of 2016

CONTACT US
Email: info@profacgen.com
Tel: 1-631-448-8149
Fax: 1-631-938-8127
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA