Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately>40% will adhere specifically after 30 minutes a
Molecular Mass
Recombinant Human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 29 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EpCAM is approximately 36 kDa due to glycosylation.