Recombinant Mouse Ctsb Protein(His tag) (MI0001CS)

Recombinant Mouse Ctsb Protein (NP_031824) (18-339aa) with C-terminus His tag was expressed in Insect cells.

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PRODUCT INFORMATION

Cat.No.

MI0001CS

Synonyms

CTSB; cathepsin B; cathepsin B1; CB;

Species

Mouse

Source

Insect cells

Tag

His tag

Form

Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Molecular Mass

36.4kDa (330aa)
28-40kDa (SDS-PAGE under reducing conditions)

AA Sequence

HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGSCWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPPCTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

BACKGROUND

Background

This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome.

References

Podgorski I., et al. (2003) Biochem Soc Symp. 70:263-276.
Roshy S., et al. (2003) Cancer Metastasis Rev. 22:271-286.

Reference Reading

1. Sigloch, Florian Christoph; Knopf, Julia Daniela; Weisser, Juliane; et al. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1863-11,2700-2709

"Cathepsin B (CTSB) is a lysosomal endo- and exopeptidase that is also secreted in high amounts by malignant and non-malignant cells. We determined the effect of CTSB on the tumor cell secretome by shRNA-mediated silencing of CTSB mRNA expression and subsequent proteomic LC-MS/MS analysis of the cell supernatants. We identified significant protein changes of 17 secreted or shed proteins."

2. Moon, Hyo Youl; Becke, Andreas; Berron, David; et al. Running-Induced Systemic Cathepsin B Secretion Is Associated with Memory Function, CELL METABOLISM, 24-2,332-340

"Here, we show that a muscle secretory factor, cathepsin B (CTSB) protein, is important for the cognitive and neurogenic benefits of running. Proteomic analysis revealed elevated levels of CTSB in conditioned medium derived from skeletal muscle cell cultures treated with AMP-kinase agonist AICAR. Consistently, running increased CTSB levels in mouse gastrocnemius muscle and plasma. Furthermore, recombinant CTSB application enhanced expression of brain-derived neurotrophic factor (BDNF) and doublecortin (DCX) in adult hippocampal progenitor cells through a mechanism dependent on the multifunctional protein P11."

3.Jiang, Yong; Woosley, Alec N.; Sivalingam, Nageswaran, et al. Cathepsin-B-mediated cleavage of Disabled-2 regulates TGF-beta-induced autophagy, NATURE CELL BIOLOGY, 18-8, 851-+

"Here we show that following TGF-beta-mediated EMT, sustained TGF-beta treatment leads to proteolytic degradation of Dab2 by cathepsin B (CTSB), loss of the mesenchymal phenotype and induction of autophagy. CTSB inhibition or expression of a CTSB-resistant Dab2 mutant maintains Dab2 expression and shifts long-term TGF-beta-treated cells from autophagy to apoptosis. We further show that Dab2 interacts with Beclin-1 to promote casein-kinase-2-mediated phosphorylation of Beclin-1, preventing Beclin-1-Vps34 interaction and subsequent autophagosome assembly."

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