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Recombinant Human CTNNB1 Protein(His-GST tag) (HI0184CL)

Recombinant Human CTNNB1 Protein (P35222) (Met 1-Leu 781) with polyhistidine-tagged GST tag at the N-terminus was expressed in Insect cells.

Size Price Qty
50ug $429.00
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PRODUCT INFORMATION

Cat.No.
HI0184CL
Synonyms
CTNNB1; catenin beta-1; beta catenin; CTNNB
Species
Human
Accession
Source
Insect cells
Tag
His-GST
Predicted N Terminal
Met
Form
Lyophilized from sterile 50mM Tris, 100mM NaCl, 2mM GSH, 10% gly, pH 8.0, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind biotinylated mouse ERBB2 in a functional ELISA.
Molecular Mass
Recombinant Human CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. It migRates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 87 % by SDS-PAGE.

BACKGROUND

Background
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).
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