Recombinant Mouse Ldha Protein (His tag)

Recombinant Mouse Ldha Protein (His tag) (MI0034LS)

Recombinant Mouse Ldha Protein (NP_034829) (1-332aa) with C-terminus His tag was expressed in Insect cells.

Size Price Qty
10ug $214.50

PRODUCT INFORMATION

Cat.No.
MI0034LS
Synonyms
LDHA; LDH-M; M-LDH; Ldh1; Ldhm; l7R2;
Species
Mouse
Source
Insect cells
Tag
His tag
Form
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Bio-activity
Specific activity is> 250 units/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37 centigrade.
Molecular Mass
37.5kDa (340aa)
28-40KDa (SDS–PAGE under reducing conditions.)
AA Sequence
MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSKLVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGVHALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRVHPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity
> 95% by SDS - PAGE

Usage Guide

Storage
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.5mg/ml (determined by Absorbance at 280nm)
Warning
For research use only!

BACKGROUND

Background
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene.
References
Rong Y., et al. (2013) Tumour Biol. 34(3):1523-1530.
Merkle S., et al. (1992) Genetics. 131(2):413-421.

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