Yeast Expression Systems(P pastoris / S cerevisiae)

Utilizing Profacgen's proprietary YeastXceed^TM Technology, selected stable and durable production strains that resemble mammalian system are employed for high-yield, high-productivity protein processing, thus dramatically reducing the associated costs and inherent time requirements.

HostOptim^TM boosts recombinant protein production per cell by increasing the protein per mRNA ratio across a range of eukaryotic production hosts. Other available recombinant production technologies typically target transcription and are limited to specific production systems. HostOptim^TM can be used to increase the production yield of your recombinant protein and can be combined with transcription enhancing or other production improving technologies.

Yeast protein expression system is the most economical eukaryotic expression system for both secretion and intracellular expression. It is ideally suitable for large-scale production of recombinant eukaryotic proteins.

Our expertise in protein expression spans both P. pastoris and S. cerevisiae expression systems. If you're looking for protein production via a fed-batch pichia processes, We have the experienced and credentialed staff to move your project forward.

Our YeastXceed^TM Technology considers promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location, which are essential for high-level recombinant protein production.

Profacgen's engineering-enhanced protein expression technologies enable a high success rate of over 95% over a wide variety of recombinant proteins, and our proprietary YeastXceed^TM technology is pushing yields even higher. Many secretory proteins that bacterial expression systems yield as insoluble or as inactive inclusion bodies can be produced successfully by our yeast expression system using native or yeast secretion signals.

We have identified DNA elements which are instrumental in increasing translation rates when included in recombinant protein expression vectors. These elements are functional across eukaryotic production platforms, including mammalian cells, yeast, and most fungal systems. Based on these elements, Profacgen has developed its proprietary HostOptim^TM technology.

Using a range of mammalian, yeast and fungal cell types, the functionality of HostOptim^TM technology has been confirmed for a variety of industry relevant platforms. Depending on initial expression levels, expression of secreted recombinant proteins can be increased several-fold, and is coupled to a similar increase in recombinant protein to mRNA ratios.