Product description
Proline content in plants to a certain extent reflects plant stress resistance. Drought-resistant varieties tend to accumulate more proline. Therefore, determining proline content can serve as a physiological indicator for drought-resistant breeding. Additionally, due to its strong hydrophilicity, proline can stabilize protoplasm colloids and metabolic processes within tissues, thereby lowering the freezing point and preventing cell dehydration. Under low-temperature conditions, increased proline content in plant tissues can enhance cold resistance, making it a physiological indicator for cold-resistant breeding as well. When plantT are extracted with sulfosalicylic acid, proline becomes free in the solution, then reacts with acidic ninhydrin upon heating to form a red substance, which has a maximum absorption peak at 520nm. The content of free proline in plants can be determined through colorimetry.
Additional Materials and Equipments Required
Spectrophotometer, 1mL glass cuvette (light path 1cm), constant temperature water bath, desktop centrifuge, pipette, glacial acetic acid, mortar, ice