Recombinant Human CXCL12 Protein(Fc tag) (HH0093CL)

Cat.No.

HH0093CL

Synonyms

CXCL12; PBSF; SCYB12; SDF 1a; SDF 1b; TLSF a; TLSF b; TPAR1; IRH; SDF1; TLSF; SDF1A; SDF1B;

Species

Human

Accession

CXCL12

Source

HEK293

Tag

Fc

Predicted N Terminal

Glu 20

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its ability to bind biotinylated recombinant human DPPIV in a functional ELISA.

Molecular Mass

Recombinant Human SDF1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 309 amino acids and predicts a molecular mass of 35.2 kDa. As a result of glycosylation, rhSDF1/Fc monomer is approximately 43 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1.