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Enzyme Activity Assay

Enzyme Activity Assay

Profacgen provides various enzyme activity assays for kinases, phosphatases, proteinases, deacetylase, peptidase, esterase, and other enzymes. Our line of well-characterized immunoassays and biochemical kits ensures accurate and reproducible results.

Enzyme is a large category of bio-molecules that catalyze various biological processes including metabolic processes, cellular signaling and regulation, cell division and apoptosis. Enzymatic reactions convert substrate molecules into chemically modified molecules products with high specificity and accuracy. These modifications include proteolytic digestion, glycolytic degradation, phosphorylation, acylation, glycosylation or oxidative and reductive processes.

As enzymes play important roles in numerous physiological activities, many of them become critical targets for diseases treatments. For example, matrix metalloproteinase (MMP) plays critical role in anti-tumor metastasis. Protein kinase B (PKB) and protein kinase C (PKC) are major drug targets for clinical treatment of diabetes. HIC and HCV viral proteinase can serve as therapeutic target for antiviral therapy. Cytochrome P450 is a vital enzyme during drug metabolism and has great influence on thermoregulation. Based on the above, enzyme activity assay can bring great significance in novel drug development and clinical therapy.

Enzyme Activity Assay Detection Methods
(Left: fluorescence method; right: colorimetric analysis)

Enzyme activity assay detection methods

Profacgen provides a comprehensive list of enzyme activity assay services for your enzyme research. Our extensive experience in the determination of enzyme activity supports the development of an ever-expanding portfolio of assays, facilitating better understanding of the enzymes’ roles in physiological processes. Some enzymes’ activity can be measured by its conversion or production of readily detectable molecules such as ATP, NADH and pyruvate. Others require specialized assays based on their enzymatic properties. For example, MMP activity is usually analyzed by zymography.

Enzyme Activity Assay Items

Classification Name
Kinase Protein tyrosinekinase (PTK)
Aurora kinase (AURK)
Janus kinase (JAK)
Mitogen-activated protein kinase
Microtubule affinity-regulating kinase (MARK)
Cyclin dependent kinase (CDK)
Polo-like kinase (PLK)
Pyruvate dehydrogenase kinase, isozyme 1 (PDK1)
Protein kinase B (AKT/PKB)
Protein kinase C (PKC)
Protein kinase D (PKD)
Ca2+/Calmodulin-dependent protein kinase (CAMK)
cAMP-dependent protein kinase A (PRKACA)
cGMP-dependent protein kinase (PRKG)
Glycogen synthase kinase (GSK)
BR serine/threonine kinase 1 (BRSK1)
Mitogen activated protein kinase (MAPK)
Phosphatidylinositide3-kinase (PI3K)
P38 stress-activated protein kinase (P38/SAPK)
JUN N terminal kinase (JNK)
Phosphatase JNK activating phosphatase 1 (JSP-1)
Vaccinia virus VH1-related phosphatase (VHR)
Protein tyrosine phosphatase (PTP)
Dual specificity phosphatase 13 (DUSP13)
Phosphatase of regenerating liver 3 (PRL-3)
CaN Calcineurin
Proteinase Matrix metalloproteinase (MMP)
Cysteinyl aspartate specific proteinase (Caspase)
20S proteasome
HIV viral proteinase
HCV viral proteinase
Other enzymes Histone deacetylase (HDAC)
Cathepsin S
Angiotensin-converting enzyme (ACE)
Butyrylcholine esterase
Acetylcholine esterase
Dipeptidase (DPP)
FTO demethylase
Monoamine oxidase (MAO)
Cyclooxygenase-2 (COX-2)
Cytochrome P450 (CYP450)

Our Advantages:

  Versatile testing methods
  High sensitivity
  Accurate and consistent results
  Strong technical support team
  Best price in the market

Please do not hesitate to contact us for more details about our enzyme activity assay service.

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