In the past decades, transgenic plants system has attracted the attention of pharmaceutical company for biopharmaceuticals production. Microalgae, also called microphytes, are polyphyletic groups ranging from prokaryotes to eukaryotes, such as Synechococcus and Chlamydomonas. It has been used for production of food, food additives, and cosmetics for a long time. Chlamydomonas reinhardtii is the most popular microalgae used in research studies. As eukaryotes, algae can perform posttranslational modifications such as glycosylation, which are often necessary for protein function. Besides, microalgae are toxin free and animal free in production of recombinant protein, which makes microalgae has a advantage in biosecurity. With the posttranslational modifications and reduced pathogenic compounds contamination, algae become more and more attractive for bio-pharmaceuticals production.
Chlamydomonas reinhardtii, as the unicellular green microalga, is the one of model organisms. In the past decades, it had been used in production of enzymes, antigenic peptides or antibodies and the application of algal system has gaining the interest in valuable biopharmaceutical production. Besides, target protein allowed to express in both nuclear and chloroplast genomes of Chlamydomonas.
Figure 1. Modes of transgene expression in a Chlamydomonas reinhardtii cell illustrated by a schematic drawing. (1) Nuclear expression and targeting of the protein to the cytoplasm. (2) Nuclear expression and secretion of the protein to the medium via the endoplasmatic reticulum and the golgi apparatus (these organelles are omitted in the drawing). The protein can be posttranslationally modified. (3) Protein expression in the chloroplast. The product is accumulated within the chloroplast; posttranslational modifications and secretion are not possible.
Short production time
Cost-effective expression systems
Easy handling and cultivation
Profacgen provides you recombinant protein generation in Chlamydomonas reinhardtii system from gene cloning to protein expression and purification. We have professional experts for study of transgenic microalgae and state-of-art protein express and purify resources. With our in-house platform, Profacgen expresses the high-value products for our customer.
Profacgen promises to work closely with our customers to provide excellent services from gene to protein, and finally helps our customer with unique biological insights.
1. Christoph Griesbeck, Iris Kobl, and Markus Heitzer, Chlamydomonas reinhardtii: A Protein Expression System for Pharmaceutical and Biotechnological Proteins. Mol Biotechnol. 2006 Oct; 34(2): 213-23.
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