Product description
Sialic acid is a component of cell membrane glycoproteins and glycolipids, located at the terminal of cell membrane glycoproteins and glycolipids, and involved in various physiological functions on the cell surface. Malignant tumors can cause significant changes in the structure and content of surface glycoproteins and glycolipids, leading to the shedding of SA into the blood and increasing serum SA levels. The measurement of SA is significant for the study of certain congenital metabolic disorders and Behcet's syndrome. Elevated SA levels are also one of the risk factors for cardiovascular diseases. SA is acted upon by neuraminidase to form N-acetylneuraminic acid, which is then converted into pyruvate and N-acetylmannosamine by N-acetylneuraminic aldolase. Pyruvate is converted into lactate and NAD+ by lactate dehydrogenase (LDH) in the presence of NADH. The concentration of SA in the sample can be determined by measuring the decrease in NADH absorbance.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, centrifuge, distilled water.