Customized Expression Service of Mutant SARS-CoV-2 Protein

Customized Expression Service of Mutant SARS-CoV-2 Protein

High rates of mutation accumulation over short time periods have been reported previously in studies of immunodeficient or immunosuppressed patients who are chronically infected with SARS-CoV-2. The patients are treated with convalescent plasma and usually also with the drug remdesivir. Virus genome sequencing of these infections reveals unusually large numbers of nucleotide changes and deletion mutations and often high ratios of non-synonymous to synonymous changes.

A terrible mutant strain of the SARS-CoV-2, B.1.1.7, was discovered in the UK. This new strain has 23 special mutations. This new strain has a mutation of N501Y in the six key interface amino acids of RBD, and a mutation of P681H occurred in the position adjacent to the Furin cutting site. The Furin protease cleavage site has a huge impact on the ability of SARS-CoV-2 to bind to host cells. The stability of the S protein after Furin cleavage is reduced, thus exposing the open domain, thereby increasing the binding affinity of the S protein to the human ACE2 receptor by 1000 times.

In addition to the obvious increase in the spread of the new strain, there are other dangers waiting to be verified.

Table 1.  Non-synonymous mutations and deletions inferred to occur on the branch leading to lineage B.1.1.7 lineage.

gene nucleotide amino acid
ORF1ab C3267T T1001I
C5388A A1708D
T6954C I2230T
11288-11296 deletion SGF 3675-3677 deletion
spike 21765-21770 deletion HV 69-70 deletion
21991-21993 deletion Y144 deletion
A23063T N501Y
C23271A A570D
C23604A P681H
C23709T T716I
T24506G S982A
G24914C D1118H
Orf8 C27972T Q27stop
G28048T R52I
A28111G Y73C
N 28280 GAT->CTA D3L
C28977T S235F

Profacgen is a state-of-the-art protein service provider, we provide custom protein services in the biological sciences. To support scientists, we integrated a platform to offer high-quality and efficient technical services, and provide customers with reliable assistance in the research of viral proteins, such as protein expression services, protein interaction analysis services, enzyme activity assay, computational protein analysis, and virtual screening.

Facing the challenge of the new coronavirus mutant, we specially provide spike mutant protein customization service. We have successfully expressed a variety of SARS-CoV-2 mutant proteins. If you do not find the mutant protein product in stock, please contact us to produce high-quality mutant protein according to your needs.


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