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Glycogen phosphorylase (Glycogen phosphorylase, GP, EC 2.4.1.1) is a key enzyme in glycogen metabolism, breaking α-1,4-glycosidic bonds from the non-reducing end of glycogen molecules to remove glucose residues and release glucose-1-phosphate, until it reaches four glucose residues before the α-1,6-glycosidic bond branching point of the glycogen molecule. GP is divided into active glycogen phosphorylase a (GPa) and inactive glycogen phosphorylase b (GPb). Glycogen degradation mainly occurs under the catalysis of GPa. This kit provides a rapid, sensitive, and simple detection method. GPa catalyzes glycogen and inorganic phosphorus to produce glucose residues, forming glycogen and glucose-1-phosphate. Phosphoglucomutase and glucose-6-phosphate dehydrogenase then catalyze the reduction of NADP+ to NADPH, which reacts with a specific color reagent to form a colored substance. By detecting the increase in the colored substance at 450nm, the GPa enzyme activity can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice.
