Recombinant Human IDO1 Protein(His tag) (HE0189IL)

Cat.No.

HE0189IL

Synonyms

IDO1; IDO; INDO; IDO-1

Species

Human

Accession

IDO1

Source

E. coli

Tag

His

Predicted N Terminal

Met

Form

Supplied as sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0.

Bio-activity

Measured by its ability to oxidize L-tryptophan to N-formylk-ynurenine. The specific activity is> 400 pmoles/min/μg.

Molecular Mass

Recombinant Human IDO1 consisting of 409 amino acids and has a calculated molecular mass of 46 kDa. It migRates as an approximately 44 kDa band in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 88 % by SDS-PAGE.

Background

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).