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Starch, a polysaccharide widely present in the roots, stems, leaves, seeds, fruits, and other tissues of plants, is hydrolyzed into glucose using an acid hydrolysis method. The glucose content is then determined using the anthrone colorimetric method, which can be converted into starch content, with the determination wavelength at 620nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, pipette, mortar and pestle, room temperature centrifuge, concentrated hydrochloric acid, concentrated sulfuric acid, and distilled water.
