3-Phosphoglycerate Assay Kit (FASQP-K017S)

Cat.No.

FASQP-K017S

Method

Spectrophotometry

Product size

48T

Product description

3-Phosphoglycerate (G3P) is oxidized by glycerol phosphate oxidase (GPO) to generate hydrogen peroxide (H2O2). H2O2 reacts with 4-aminoantipyrine to form a red quinone compound with a characteristic absorption peak at 510nm. By measuring the absorbance at 510nm, the glycerol content can be determined.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), pipette, centrifuge, mortar.