We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our
Privacy Policy
This method uses ethyl blocked p-nitrophenyl maltotrioside as a substrate, which is hydrolyzed by α-amylase into ethyl maltose (Et-Gx) and p-nitrophenyl maltose (Gy-pNP). Gy-pNP can be further hydrolyzed by α-glucosidase into glucose and p-nitrophenyl (pNP)-glucoside. The final result of the amylase hydrolysis reaction and α-glucosidase action is the production of free p-nitrophenol (p-NP). p-NP has a characteristic absorption peak at 405nm, and its absorbance change is positively correlated with the activity of α-amylase in the sample.
