Choline Acetyltransferase (ChAT) Activity Assay Kit (FASAC-K014M)

Cat.No.

FASAC-K014M

Method

Microplate-based method

Product size

48T

Note

Provide microplate

Product description

Choline acetyltransferase (ChAT, EC 2.3.1.6) is the synthetic enzyme of acetylcholine (Ach) and regulates the metabolism of Ach. Ach is the main excitatory neurotransmitter that regulates the movement of esophageal smooth muscle. The determination of ChAT is based on the substrates acetyl-CoA and choline. Under the action of ChAT, acetylcholine and CoA are generated, and the product reacts with the color reagent to have an absorption peak at 412nm, thereby calculating the activity of ChAT. The enzyme-catalyzed reaction equation is: acetyl-CoA + choline = CoA + O-acetylcholine.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, balance, low-temperature centrifuge.