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Dehydrogenases (DHA) are a class of enzymes that catalyze the oxidation-reduction reactions of substances. Traditional methods use triphenyltetrazolium chloride (TTC) as a hydrogen acceptor for dehydrogenases, but the resulting colored substance, formazan, is insoluble in water, making the operation troublesome and the sensitivity low. This kit provides a simple, sensitive, and rapid method using a modified nitro-tetrazolium salt as a hydrogen acceptor. The resulting orange-yellow formazan substance is soluble in water, and the absorbance at 460nm is measured to determine dehydrogenase activity.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, balance, constant temperature incubator or water bath, pipette, low-temperature centrifuge
