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Glutamine amidotransferase (GLS, EC 3.5.1.2) is a type of amidase that catalyzes the hydrolysis of glutamine to glutamate and ammonia, playing a significant regulatory role in nitrogen metabolism, especially in regulating free ammonia levels and urea metabolism. GLS catalyzes the hydrolysis of glutamine to L-glutamate and ammonia. Ammonia, in a strong alkaline environment, reacts with hypochlorite and phenol to form the water-soluble dye indigo blue, which has a stable solution color. It has a characteristic absorption peak at 630nm, and by detecting the rate of increase of ammonia, the enzyme activity can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.
