Glycerol Assay Kit (Enzymatic Method) (FASQG-K016M)

Cat.No.

FASQG-K016M

Method

Microplate-based method

Product size

96T/500T

Note

Provide microplate

Product description

Glycerol, stored in fat cells, is one of the final products of triglyceride metabolism. In production and daily life, glycerol can be used as a solvent, lubricant, pharmaceutical, and sweetener. Glycerol is catalyzed by glycerol kinase (GK) to form glycerol-1-phosphate (G-1-P). G-1-P is oxidized by glycerol phosphate oxidase (GPO) to generate hydrogen peroxide (H2O2), which reacts with 4-aminoantipyrine to form a red quinone compound with a characteristic absorption peak at 510nm. By measuring the absorbance at 510nm, the glycerol content can be determined.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, centrifuge, mortar, distilled water.