Homocysteine (HCY) Assay Kit (Enzymatic Recycling Method) (MBMQH-K035M)

Cat.No.

MBMQH-K035M

Method

Microplate-based method

Product size

48T/96T

Note

Provide microplate

Product description

Oxidized homocysteine is reduced by tris(2-carboxyethyl)phosphine (TCEP) to form free HCY, which reacts with the substrate in a cyclic amplification, simultaneously producing adenosine. Adenosine is immediately hydrolyzed into ammonia and hypoxanthine, and ammonia converts NADH to NAD+ under the action of glutamate dehydrogenase. By measuring the decrease in NADH at 340nm, the HCY content can be calculated.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, centrifuge, distilled water.