Product description
Isocitrate lyase (ICL, EC 4.1.3.1) is one of the key enzymes in the glyoxylate cycle, mainly found in plants and microorganisms. During the germination of oilseed crops, fatty acids are converted into carbohydrates through β-oxidation and the glyoxylate cycle. Therefore, measuring ICL activity is significant for understanding the metabolic pathways and material transformation in oilseeds, as well as the vitality of seeds. ICL catalyzes the decomposition of isocitrate to form one molecule of succinate and one molecule of glyoxylate. Glyoxylate forms a brown color with dinitrophenylhydrazine (DNPH) under alkaline conditions, and the depth of color is directly proportional to the content of glyoxylate. Thus, the content of glyoxylate can be determined colorimetrically, and the activity of ICL can be calculated. The enzyme-catalyzed reaction is: isocitrate = succinate + glyoxylate.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, low-temperature desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.