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α-Ketoglutarate dehydrogenase (α-KGDH, EC 1.2.4.2) is one of the key regulatory enzymes of the citric acid cycle and is widely present in animals, plants, microorganisms, and cultured cells. It can catalyze the conversion of α-ketoglutarate, NAD+, and coenzyme A into succinyl coenzyme A, carbon dioxide, and NADH. The enzyme activity is determined by measuring the increase rate of NADH at 340 nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.
