Leucine Aminopeptidase (LAP) Assay Kit (NMSAL-K024M)

Cat.No.

NMSAL-K024M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Leucine aminopeptidase (LAP, EC 3.4.11.1) is a protease that hydrolyzes peptide chains with leucine at the N-terminus and is widely present in tissues such as the liver, kidneys, and pancreas, especially abundant in the liver. LAP decomposes L-leucyl p-nitroanilide to produce p-nitroaniline, which has a maximum absorption peak at 405nm. By measuring the rate of increase in absorbance, the activity of LAP can be calculated.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, desktop centrifuge, pipettes, balance, mortar, ice, and distilled water.