Product description
NAD-isocitrate dehydrogenase (NAD-IDH, EC 1.1.1.41) is widely present in the mitochondria of animals, plants, and cultured cells. It catalyzes the conversion of isocitrate to α-ketoglutarate in the citric acid cycle, simultaneously reducing NAD+ to NADH. It is one of the rate-limiting enzymes in the citric acid cycle, and its catalyzed reaction is one of the main sources of cellular NADH. The enzyme activity of NAD-IDH can be determined by the increase in light absorption at 340nm due to the reduction of NAD+ to NADH.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, low-temperature centrifuge, pipette, mortar and pestle, ice, and distilled water.