Mitochondrial Respiratory Chain Complex II/Succinate-CoQ Reductase Assay Kit (REMAM-K033S)

Cat.No.

REMAM-K033S

Method

Spectrophotometry

Product size

24T/48T

Product description

Mitochondrial Complex II (EC 1.3.5.1), also known as succinate-CoQ reductase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It catalyzes the oxidation of succinate to fumarate, and the reduction of the prosthetic group FAD to FADH2, which further reduces oxidized CoQ to reduced CoQ. This is the first step in controlling the succinate oxidation respiratory chain reaction and is also key to the oxidative phosphorylation energy production process. The reduced CoQ produced by Complex II can further reduce 2,6-dichlorophenolindophenol, reducing the absorbance of this substance at 600nm. The enzyme activity of Complex II can be obtained by measuring the decrease rate at 600nm.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), pipette, low-temperature desktop centrifuge, mortar and pestle, anhydrous ethanol, ice, and distilled water.