NAD Kinase (NADK) Assay Kit (REMAN-K011S)

Cat.No.

REMAN-K011S

Method

Spectrophotometry

Product size

48T

Product description

NADK (EC 2.7.1.23) is widely present in animals, plants, microorganisms, and cultured cells, and is the only enzyme found in organisms that can catalyze the phosphorylation of NAD+ to NADP+. Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H). NADK catalyzes the phosphorylation of NAD+ to NADP+; then, under the action of 6-phosphogluconate dehydrogenase and 6-phosphogluconate, NADP+ is reduced to NADPH. The enzyme activity is determined by measuring the increase rate of NADPH at 340 nm.

Additional Materials and Equipments Required

UV spectrophotometer, 1mL quartz cuvette (optical path 1cm), low-temperature desktop centrifuge, pipette, mortar and pestle, and distilled water.