Product description
Malate enzyme is an important enzyme widely present in animals, plants, and bacteria. It is a key enzyme in malate metabolism. In recent years, the activity of plant ME has been widely measured and has become a hot topic in antioxidant research. This enzyme requires the participation of coenzyme factors and is divided into NADP-ME (EC 1.1.1.40) and NAD-ME (EC 1.1.1.38) based on different coenzyme factors. The main function of ME is to catalyze the oxidation and decarboxylation of malate to produce pyruvate and CO2, as well as the reduction of NAD(P)+. NADP-ME catalyzes the reduction of NADP+ to NADPH. This kit can determine the enzyme activity of NADP-ME by measuring the increase rate of NADPH at 340 nm.
Additional Materials and Equipments Required
UV-Vis spectrophotometer, 1mL quartz cuvette (light path 1cm), desktop centrifuge, water bath, pipette, distilled water.